Toxic Effect of Ladosulfan-Pesticides on Biochemical Indices of Clarias Gariepinus (Burchell 1822) Juveniles

Abstract

Helen Ogochukwu Nwamba, Christopher Didiugwu Nwani, Victor Stephen Njom, Emeka Ezenwa Ogamba

ladosufan is an insecticide extensively used in agriculture for pest control. The 96h LC50 estimated from probeit analysis was 1.89mg/L. Sub lethal concentrations of 1/10th (0.19 mg/L),1/5th (0.49mg/L) and 1/2th (0.95mg/L) of LC50 were used to determine the effects of the ladosulfan -pesticide on biochemical parameters at day 1, 7, 14 and 21exposure periods. Water quality test was determined on pH, temperature, dissolved oxygen, hardness and alkalinity. The results show that biochemical parameters viz; Total Protein, Alkaline Phosphatase ALP, Alanine Transferase ALTAspartate Amino Transferase (AST) concentrations increased significantly (P ≤ 0.05) in time and concentration dependent manner. The biochemical parameters concentration had higher values in treated samples than control. The total protein increased from (8.80±0.002b -9.20±0.103b) at 0.19 mg/L, (9.20±0.102a –9.70±0.103c) at 0.48 mg/L and (9.27±0.152a –9.80±0.103c) at 0.95 mg/L of ladosulfan when compared with the control (7.67±0.931c –9.06±0.763c ); Alkaline Phosphatase increased from (60.00±0.002a -86.00±1.001b) at 0.19 mg/L, (62.00±1.002b –88.00±1.001c) at 0.48 mg/L and (65.00±1.001a –92.00±1.002d) at 0.95 mg/L of endosulfan when compared with the control (40.00±1.002a –53.67±5.511a) ; Alanine Transferase also increased from (30.00±1.002a -40.00±1.001c) at 0.19 mg/L, (35.00±1.002a –54.00±1.001a) at 0.48 mg/L and (38.00±1.002b –89.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (22.33±2.083c –27.33±2.882b) ; Aspartate Amino Transferase increased from (64.00±1.002b -87.00±1.001c) at 0.19 mg/L, (66.00±1.002c –88.00±2.001d) at 0.48 mg/L and (68.33±0.582c –90.00±1.001d) at 0.95 mg/L of ladosulfan when compared with the control (63.00±2.002b –68.00±1.001b) ; respectively. The results indicate that commercial formulation of ladosulfan is toxic to C. gariepinus, the insecticide should be prudently used in both terrestrial and aquatic eco-systems to avoid eco-toxicological hazards.

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