Three Selected African Medicinal Plants Induce Anticancer and Phytochemical Properties in Prostate Cancer Cell Line

Abstract

Montasir Ahmed Elnour

Modern pharmacology, however, relies on refined chemicals - either obtained from plants, or synthesized. This work investigated the anticancer, antioxidant and Phytochemical properties of three Sudanese Medicinal Plants commonly used as anti-inflammatory and anti-tumor. L. inermis leaves, M. piperita leaves, G. barbadense leaves. All the plant parts were extracted using 80% methanol, the anticancer activity was examined by using MTT assay against PC3 (prostate cancer) cell lines and determine their antioxidant by testing metal chelating activities, cytotoxicity using - (4, 5-Dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), filter and kept in dark, prepared freshly. The extract L. inermis leaves has shown very high activity against PC3, the extract M. piperita leaves has shown good activity against PC3 and G. barbadense leaves has shown none active anti- PC3 with IC50 values 48,4 >64,6 and >100 μg/ml respectively. Phytochemical screening of L.inermis leaves indicated presence of cumarin, saponins in trace amount, flavonoids, triterpenes in moderate amount, and sterols, tannins in high amount, alkaloids and anthraquenones were not detected. Phytochemical, screening of the M. piperita leaves indicated presence of cumarin, saponins in trace amount, flavonoids, triterpenes in moderate amount, sterols, and tannins in high amount, alkaloids, and anthraquenones were not detected. Phytochemical screening of G. barbadense leaves indicated presence of alkaloids, flavonoids, saponins in trace amount, triterpenes in moderate amount, tannins in high amount, and cumarin, sterols, anthraquenones were not detected. All the three plants extracts were examined for their metal chelating activity at concentration 50 µg/mL.

All the examined plant methanol extrats showed different levels of inhibition % M. piperita leaves 52.46 µg/m, L. inermis leaves 26.27 µg/m, G. barbadense leaves 11.07 µg/m.

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