Simultaneous Equation Method for the Estimation of Caffeine and Pioglitazone HCL by UV-Visible Spectrophotometry

Abstract

Mohd Anas Shaikh, Jaya Agnihotri and Khan Saba

A simple, rapid, accurate, economical and precise UV/VIS method has been developed and validated. Choices of a common solvent were essential so various solvent ranges including methanol, ethanol, acetonitrile, and 0.1 N HCL, and various concentrations ranges of various buffers were analyzed. Hence 0.1 N HCL was selected as a solvent for the proposed method. Caffeine and Pioglitazone HCL showed maximum absorbance at 273 and 220 nm respectively. Both drugs obey Beer Lambert’s law in the concentration range of 3-18 μg/mL for Caffeine and Pioglitazone HCL respec- tively. The LOD and LOQ were found to be 0.5476 μg/mL and 1.6594 μg/mL for Caffeine respectively. For Pioglitazone HCL the LOD and LOQ values were found to be 0.6111 μg/mL and 1.8519 μg/mL respectively. The method was quanti- tatively evaluated in terms of linearity, precision, precision, LOD, LOQ and recovery. The method is simple, convenient and suitable for the analysis of Caffeine and Pioglitazone HCL in bulk drugs.

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