Serum Osteoprotegrin, Total Srankl and Their Determinants in Adolescents with Type 1 Diabetes

Abstract

Mieczys�?aw Szalecki, Elżbieta Wierzbicka, Edyta Szymanska, Marta Wysocka-Mincewicz, Edyta Jab�?o�?ska-Wypustek and Pawe�? P�?udowski

Aim: The aim of the study was to evaluate the levels of osteoprotegerin (OPG), receptor activator of nuclear factor –κB ligand (s-RANKL), OPG/sRANKL ratio and sRANKL/OPG ratio in adolescent patients with type 1 diabetes mellitus (T1DM), and to assess their correlation with the following factors: patients’ gender, age, metabolic control, age of the diagnosis and duration of the disease.

Patients and Methods: 60 T1DM patients (32 girls and 28 boys, mean±SD age: 15.0±1.9 years, diabetes duration: 5.1±3.9 years, age of the diagnosis: 9.9±3.9 years, HbA1c: 7.9±1.4%) and 18 healthy matched controls were included. Osteoprotegerin and total sRANKL (free and bound sRANKL) were measured by ELISA (enzyme-linked immunosorbent assay).

Results: There were no statistically significant differences in serum OPG levels between patients and controls (51.56±12.05 vs. 50.98±13.55pmol/L, p=0.84. No correlation betweengender and OPG levels both in the study and control group has been reported, although OPG levels were significantly lower in diabetic boys (2.59±0.67 pmol/L) than in control boys (3.30±1.01 pmol/l) (p = 0.031). Tetryl analysis (qualifications) dependent on OPG levels has demonstrated statistically significant correlation between the study group and clinical factors such as: gender, age of the diagnosis and duration of the disease, but not with the age. Only the tendency toward correlation between OPG and metabolic control of diabetes (p=0.093) has been observed. No statistically significant differences in sRANKL levels between the study group and controls has been identified. In patients with T1DM no correlation between RANKL levels and clinical factors such as gender, duration of the disease, age of the diagnosis and metabolic control has been reported. Only negative correlation between RANKL level and patients’ age (p = 0.002) has been observed. No correlationbetween OPG and RANKL levels has been demonstrated. No statistically significant differences in OPG/RANKL ratios between the study group and controls has been reported, the only significant difference in these ratios was observed between control females and males (p = 0.019), but not in the study group. A positive correlation between OPG/RANKL ratio and OPG level with the age has been demonstrated in the study group

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