Reconstruction of Cardiac Images using the Confocal Laser Scanning Microscope

Abstract

Abdallah Hamed and T Al-Saeed

We suggested a cardioid aperture in the processing of cardiac images using the confocal laser scanning microscope (CSLM). We calculated the Point Spread Function (PSF) corresponding to the cardioid aperture and the cardiac images by using the FFT algorithm. Then, we studied the effect of the image rotation on the PSF. In addition, we computed the autocorrelation corresponding to the cardiac images and cardiac aperture and compared them with the autocorrelation of the uniform circular aperture. Also, we computed the cross-correlation of aligned and rotated cardiac images. Finally, we imaged the cardiac images using the confocal scanning microscope provided with the circular and cardioid shapes of apertures. The MATLAB code is used in the formation of all images and plots.

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