Qualitative Analysis of Cruciferous Vegetable Extracts Useful for Estrogen Metabolism for Diindolylmethane (DIM)

Abstract

Odimegwu, Joy Ifunanya, Nkemehule Florence Ezinwa and Omotuyi Oyinkansola Elizabeth

3,3′-Diindolylmethane (DIM) is a compound derived from the digestion of indole-3-carbinol, found in Cruciferous vegetables (Broccoli, Cabbage, Cauliflower,) which promotes Estrogen metabolism in females. It has been known to help in the reduction of heavy blood flow during menstruation especially in females with uterine fibroids. Dim-plus® a herbal supplement contains Vitamin E, DIM, Phosphatidly choline, Spinach, Cabbage, and Broccoli powder. The purpose of this research is to extract and identify DIM present in Broccoli, Spinach and Cabbage obtained in local markets in Lagos, Nigeria and compare it with the reference standard DIM-plus®. Identification of compounds qualitatively by TLC showed different Rf values which were compared with the reference to identify compounds with similar Rf values. Extracts were subjected to HPLC analysis to confirm the presence of DIM in the Cruciferous vegetables. Based on the TLC and HPLC analysis it was confirmed that the vegetables crude extracts contains detectable amounts of DIM.

PDF