Predominance of Catalase Gene Mutations in Type 2 Diabetes Mellitus High-Risk Population

Abstract

Syeda Nuzhat Nawab, Sanober Kafeel, Nazia Hassan, Afsheen Arif, Zehra Hashim and Ashar Fawwad

Objective: To determine the-role of CAT -21-A/T (rs7943316) and -262 C/T (rs1001179) genetic/variants/in the susceptibility of type 2 diabetes mellitus (T2DM). The study also explored the likelihood of clinical and anthropometric-associated variations in T2DM.

Methods: This case-control study comprised of total n=400 subjects categorized into two/groups: T2DM (n=200) and controls (n=200). Genotyping of variants/was-carried-out-by allele-specific (AS) and RFLP-PCR/based/strategies.

Results: Distribution of genotypes revealed significant differences in both variants of CAT gene promotor. In CAT -21 A/T variant, the frequency of the mutant T/T genotype was higher in the T2DM group (56.0%) as controls (48.5%). While, in CAT -262 C/T variant, the frequency of heterozygous genotype C/T was significantly higher in diseased patients (64%)/ compared/to/controls (33%). Statistical association analysis demonstrated the significant implication of both CAT variants in the pathogenicity of T2DM (p<0.001). A significantly higher trend of systolic blood pressure, glucose, triglycerides, and LDL cholesterol levels was observed in T2DM patients (p<0.01). Whereas, plasma levels of HDL cholesterol were found to be within range in controls (p<0.001).

Conclusions: CAT variants may be regarded as potential biomarkers for the prognosis of T2DM. CAT (rs7943316) variant was involved in the modulation of risk associated with T2DM. While CAT (rs1001179) variant was involved in the development of resistance against the predisposition of T2DM.

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