Potential Use of Lipid Waste Industrial Residues for Lipase, Phospholipase and Bio surfactant Production Using a Newly Isolated Bacillus Safensis Strain

Abstract

Faten Abdeli, Ghayth Rigane, Ridha Ben Salem, Sami Aifa and Slim Cherif

The aim of this work was to produce lipases by a newly bacterial strain using as substrate, reach lipid waste by products from oil refining or commercially soap industry. For the fermentation tests, two substrates were investigated to produce lipases: soap stock (solid lipid waste from an oil refining industry) and glycerin (liquid lipid waste from soap industry). The higher level lipases production was obtained with the soap stock as the sole carbon source. Different parameters such as pH, temperature of the medium and incubation time were optimized. A correlation was also obtained between detected lipolytic activity and reduction of surface tension in the culture medium. The surface tension decreased from 50 to 25.7 mN/m indicating that biosurfactants were produced in the culture medium. As soap stock contains phospholipids molecules, this by product also enhances phospholipases production by the newly Bacillus safensis strain.

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