Potential Disease Hazards of Petrolium Hydrocarbons Contamination in Gafrarium Pectinatum (Linnaeus, 1758) From Egyptian Lake Temsah with Emphasis On Gafrarium DNA Parcoding

Abstract

Iman MK Abumourad, Taghreed B Ibrahim, Soliman WS and Elgendy MY

Assessment of petroleum hydrocarbon (PHC) contamination was carried out in water, sediments and the bivalve Gafrarium pictinatum as a part of monitoring program work. DNA barcoding was applied on G. pictinatum using Cytochrome c oxidase subunit 1 (COX1); accession no. HQ703080.1. assessment of disease risk probability due to PHC contamination using comet assay in G. pectinatum gills was studied. Results revealed that the value of dissolved petroleum hydrocarbons was 44 mg/l in water of the investigated area, 365.5 mg/kg in sediment and 135.2 mg/kg and in G. pictinatum tissue. Phylogeny of the collected bivalves was confirmed as Gafrarium pictinatum using COX1 representative primers, PCR, sequencing and blast tool on the NCBI. TPH caused DNA damage in G. pictinatum, the value of the damage differed from location to another which may be attributed to the level of hydrocarbons pollution. This study provides a basis for studying hydrocarbon contamination in marine environment.

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