Nano-Biotechnology: Developing Nano-silver coated cotton fabrics by means of biosynthesis of silver nanoparticles using Aspergillus terrues strain (MTCC9618) against staphylococcus aureus

Abstract

Abaysew Ayele Flif, Rita Singh Majumdar

Biosynthesis of silver nanoparticles, especially fungal mediated method is given attention in the development of new drugs for resistance pathogens, molecular diagnosis, drug delivery therapy and in catalytic sensor due to its cost effective, none toxicity and eco-friendly. The present study focused on the fungal mediated biosynthesis of silver nanoparticles using Aspergillus terrues strain (MTCC 9618). The synthesized nanoparticles was monitored by spectrophotometer SEM, XRD and its band gap was determined by Tuac equation. After while the fungal crude cells was exposed to 5mM silver nitrate the reduction reaction was recorded according to red shift colorchange. Based on ultra violet spectrophotometeran absorbance was recorded in a distinct pick around 430nm - 450nm and also the band gab was determined using Tuac equationsuch that 2.08eV, 2.02eV, 2.0eV and 1.96eV at about 10min, 30min, 12h and 24h respectively. The AgNPs coated cotton fabrics was developed through direct exposed to extracellular metabolites and 100ppm colloidal solution of AgNPs. The antimicrobial efficacy of the synthesized AgNPs coted cotton fabrics against gram positive Hospital staphylococcus aureus pathogenstrains was conducted by disk diffusion assay. In which the antimicrobial efficacy of coated cotton-Ag against human pathogens was proofed how the staphylococcus aureus had susceptible too and (16mm) zone of inhibition was recorded. Based on disk diffusion assay at 10ug/mL minimum inhibition concentration (MIC)10.5 mm inhibition zone was noted consequently, this study accomplished that Aspergillus terreus strain mediated biosynthesis of silver nanoparticles is cost effective, time saving, eco-friendly and small spherical (<10nm) had produced against to Physio-chemical means. The bio-synthesized silver nanoparticles cotton fabrics publicized that a higher efficacy of antimicrobial activity against staphylococcus aureus and the result was considerable suggested in widely range used in textile and pharmaceutical industries to enrich durability, strength, quality of products against a clinical pathogens application as well bad odor and spoilage of dusts from fabrics.

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