Naked-Eye Sensing of SARS-CoV-2 Utilizing Nanozymatic Nanoassays

Abstract

Saeed Reza Hormozi Jangi

The first clinical diagnosis of the new infectious disease, COVID-19 was reported on December 31, 2019. The origin of this new infectious disease is a new generation of coronavirus, i.e., SARS-CoV-2. Since, the exploration of this concept, several methods have been developed for the diagnosis of COVID-19 via the detection of SARS-CoV-2. Among different methods, the nanozyme-based colorimetric sensors have attracted good attention due to the naked-eye response and simple procedure. Hence, the aim of this review is a quick overview of the nanozyme-based sensing and detection methods for early diagnosis of COVID-19. The main basis of these sensors is the detection of color variation of a nanozyme-mediated oxidation reaction in the presence and the absence of antigens of COVID-19. In this review article, we aimed to review the recent nanozyme-based colorimetric sensors for the detection of SARS-CoV-2 to provide a brief comprehensive insight into naked-eye sensing of SARS-CoV-2. The colorimetric nanozyme-based sensing of SARS-CoV-2 is based on the interaction of nanozymes with SARS-CoV-2 nucleocapsid protein, spike (S1) protein of SARS-CoV-2, SARS-CoV-2 phosphoprotein, SARS- CoV-2 RNA, or receptor of CD147 and then probing the color change of the nanozyme-mediated oxidation of peroxidase substrates. The progress or inhibition of color intensity of the reaction system is then assigned to the presence of SARS- CoV-2 and consequently positive result of the COVID-19 test.

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