Methanol Extract of Seeds of Datura Stramonium L. Show Antioxidant Activities, Inhibit MCF-7 Cell Proliferation

Abstract

Antioxidant, cytotoxicity, MCF7, MTT assay

Background: Datura stramonium besides having several names, is commonly dubbed as thorn apple and jimsonweed weed, belongs to the family of Solanaceae and is found in many regions throughout tropical and subtropical regions. Datura seeds and leaves are used as antiasthmatic, antispasmodic, hypnotic and narcotic. Externally, the jimson seeds are used in treating of fistulas and abscesses. Datura has been used in Ayurveda for asthma symptoms where jimson weed’s leaves are smoked in cigarette or pipe.

Objectives: Present study investigates the antioxidant and antiproliferative activities of MEDS. Materials and Methods: The antioxidant assays such as DPPHË? radical, superoxide radical, ABTSË?+ radical cation, OHË? radical scavenging assays, Phoshomolybdenum reduction and Fe3+ reducing power assays were investigated. Thin layer chromatography was performed to find out compounds present in the methanol extract. In vitro cytotoxic activity for MCF7 (breast cancer) cell line was too studied by MTT reagent assay method.

Results: IC50 values of DPPHË? radical, superoxide radical, ABTSË?+ radical cation, OHË? radical scavenging assays were 35.26, 10.50, 49.36 µg/mL concentration respectively. TLC analysis showed the presence of compounds with Rfvalues of 0.76 and 0.58 in the methanol extract of D. stramonium.The cytotoxic activity for MCF7 cell line was 66.84% at 500 µg/mL concentration of methanol extract.

Conclusion: There is a potential of MEDS as a source of Antioxidants and anti-proliferative activities. Further research work required to isolate active compounds for clinical therapeutics.

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