Isolation and Screening of Antagonistic Actinomycetes For Potential Application in The Control of Pathogenic Bacteria in Contaminated Waste

Abstract

Kamal Dahdah, Heidar Nourine, Amel Boughambouz, Sarra Sebti, Laid Bouchaala and Elhafid Nabti

Organic wastes for agricultural use represent a real agronomic interest but also a risk for public and animal health. Actinomycetes present a potential source of bioactive compounds with multiple applications. Among the 88 isolates of actinomycetes obtained from different samples, we selected a strain identified, thanks to macroand microscopic characters, as Streptomyces sp. SCM21. This strain showed these potentialities to produce antibacterial metabolites against the pathogenic bacteria tested (S. aureus, B. cereus and S. enteritidis) when cultivated in liquid and solid fermentation. Solid-state fermentation was conducted using sewage sludge as substrate. This is the first time that sewage sludge has been used to produce antibacterial metabolites by a strain of actinomycetes in solid-state fermentation. In addition, solid-state fermentation moistened with distilled water gave better antibacterial activity with good sporulation of Streptomyces sp. CSM2 1 . Thus, the product of solidstate fermentation can be used in the management of contaminated waste to control pathogenic bacteria.

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