Immunological Exploration of Primary Metabolite Extracted From Aqueous Stem Extract of Caralluma Fimbriata

Abstract

Amit Gupta and Bharat Shinde

Objective: The objective of our study is to determined its immunological property of primary (protein) metabolite extracted from aqueous stem extract of Caralluma fimbriata against specific protein antigens (lactoferrin and Ovalbumin, OVA) pertaining to determine B cell production in vitro by ELISA (enzyme linked immunosorbent assay) and also measured T cell production in vitro using immature bone marrow cells of mice exposed with Incomplete freunds adjuvant (IFA) and Concanavalin (Con) A.

Methods: For these studies, estimation of protein from stem extract of Caralluma fimbriata were determined and also determining the total cellular (protein) content in immature bone marrow cells along with variable concentration of Caralluma fimbriata in presence or absence of IFA. In addition, lactoferrin (100 µg/well) and weak antigen i.e. ovalbumin (OVA; 100 µg/well) pertaining to antibody (IgG) production were determined in vitro through Elisa and also measured its proliferative response using Con A (2.5 µg/ml).

Results: The results showed that aqueous stem extract of Caralluma fimbriata showed the presence of protein (0.698 mg/ml; 10 µl) content and also showed the enhancement in total cellular (protein) content in presence or absence of IFA which is determined through NanoDrop method. In addition, this aqueous stem extract showed enhancement in anti-lactoferrin and anti-OVA IgG titre in vitro at higher doses as compared to control but there is sudden decline in bone marrow cell proliferation containing Con A at higher doses.

Conclusion: Our data suggest that aqueous stem extract of Caralluma fimbriata may help to raise antibodies in vitro against lactoferrin and OVA but sudden decline in Con A proliferative response in bone marrow cells. In other words, aqueous stem extract containing primary metabolite of Caralluma fimbriata could be a potent immune enhancer of B cells in vitro but inhibitor of T cells at higher doses.

PDF