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Biomedical Science and Clinical Research(BSCR)

ISSN: 2835-7914 | DOI: 10.33140/BSCR

Impact Factor: 1.7

Histopathological Changes Associated with Surgically Created Open Skin Wound Healing and Pancreatic Structure of Alloxan-Induced Diabetic Rabbiits

Abstract

Onah, J. A, Fadason, S. T, Abidoye, E. O, Kadima, K. B and Abalaka, S. E

The study evaluated the histopathological changes associated with the skin wound healing of alloxan-induced diabetic New Zealand White (NZW) rabbits along with the pancreatic cellular responses. Sixteen adult rabbits of either sex, weighing 1.8 - 3.2 kg were used for the study. They were divided into four groups. Group A was designated Non-Diabetic and No Wound and served as control. Group B was Diabetic and No Wound, whereas group C was Non diabetic and Wounded and group D was Wounded and Diabetic. Diabetes induction was accomplished by administration of 100 mg/kg of alloxan monohydrate twice, 72 hours apart in groups B and D only. A 3 cm2 excisional skin wound was created at the dorsum of each of the rabbits in groups C and D. Skin tissue samples (wounded sites) and pancreas were harvested from twoeuthanized rabbits in each group on day 28 post induction of diabetes for histopathologic examinations. Diabetes was induced in groups B and D on day 3 post treatment. Histopathologic findings in the diabetic rabbits included hyperkeratosis, acanthosis, poor fibrogenesis of the injured skin site, as well as the necrosis and mononuclear cellular infiltration of the islet of Langerhans of the pancreas. In conclusion, alloxan monohydrate administration created a suitable diabetic model rabbit. Diabetes mellitus caused delayed skin wound healing evidenced by hypercellularity and fibrous tissue proliferation, pancreatic necrosis and cellular infiltration of the islets of Langerhans in the diabetic rabbit.

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