Enhancing Effect of Epigallocatechin-3-Gallate (EGCG) on Liver Antioxidant Activity in Mice Exposed to Cardiovascular Disease Risk

Abstract

Emmanuel Banzubaze, Julius Mulindwa, Silver Ochwo, Eddie Wampande.

Objective: Epigallocatechin-3-gallate (EGCG) is a fundamental polyphenol compound present in green tea. Green tea has been credited for centuries with providing significant health benefits. The aim of this study was to test whether the antioxidant component of EGCG can enhance hepatic antioxidant enzyme activity and consequently increase endogenous antioxidant defense in mice exposed to cardiovascular disease. Groups of male mice were subjected to different diet (standard fat diet, high fat diet and low-fat diet), exercise (voluntary, chronic and sedentary lifestyle) and EGCG supplementation. EGCG powder was dissolved in 4 ml of drinking water to deliver a daily dose of 30 mg/kg.Mice were anesthetized with isoflurane (Abbott, Cham, Switzerland) 2% (v/v) in a 20% O2 and 80% air mixture by inhalation in a closed container, then euthanized by manual cervical dislocation and were put on the animal's bed to collect liver tissue for analysis of antioxidant enzyme activities.We compared the variation in antioxidant enzyme activity between the control and treated groups using one-way analysis of variance (ANOVA) forp-value �? 0.05% was considered statistically significant.

Results: In this study we showed that mice on a high-fat diet and sedentary lifestyle had significantly decreased superoxide dismutase 1 (p-value = 0, 001 after diets treatment, p-value = 0, 009 after exercise treatment), glutathion peroxidase 1 (p- value = 0, 016 after diets treatment, p-value = 0, 0022 after exercise treatment), catalase (p- value = 0, 019 after diets treatment,p-value = 0,005 after exercise treatment) and thioredoxin reductase 1(p- value = 0,000 after diets treatment, p-value = 0,027 after exercise treatment) activities. EGCG enhanced the activity of antioxidant enzymes in mice subjected to high fat diet or subjected to sedentary lifestyle.

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