Effect of A New Type of Drug Supplement on Epileptic Seizure Induced By Pilocarpine and the Amount of Nitric Oxide Produced by Vascular Epithelial Cells as a Trigger for Migraine Headache
Abstract
Azam Bayat Aref Khalkhali Ali Reza Mahjoub
The present study aims to investigate the effect of a new type of dietary supplement on epileptic seizure induced by pilocarpine and the amount of nitric oxide produced by vascular epithelial cells as a trigger for migraine headache. New healthy and live drug supplement were synthesized by a green route. This organic biomaterial was named NBS. Concentrations of the drug supplement were prepared by dissolving dry powder in saline phosphate-buffered. In recent years, NO has been suggested as one of the factors involved in migraine headache. Nitrite concentration was also measured as an indicator of nitric oxide production by Griess method. In order to induce seizure, on 25th day after birth, each puppy was injected into control and treatment groups with healthy and live diet in 150 mg/ kg subcutaneously. The behavior of each rat was recorded by a digital camera and two observers. It can be stated that this supplement causes a significant reduction in the level of 5% concentration of nitric oxide compared to the control group (p≤0.05). Also, 15 mg/ kg of the Dapsone increases the duration of seizure from medication to onset of seizure, while, using the new NBS healthy and live diet significantly increases this time compared to the Dapsone recipient group. The new healthy and nutritious drug can significantly reduce the incidence of migraine headaches and would prevent the onset of a seizure.