Culture of Animal Cell
Abstract
Sudha Bansode
Cell culture has become an indispensable technology in many branches of the life sciences. It provides the basis for studying the regulation of cell proliferation, differentiation and product formation in carefully controlled conditions, with processes and analytical tools which are scalable from the level of the single cell to in excess of 10 kg wet weight of cells. Cell culture has also provided the means to define almost the entire human genome, and to dissect the pathways of intracellular and intercellular signaling which ultimately regulate gene expression. Roller bottle culture is considered the first scale-up step for anchorage-dependent cells from stationary flasks or bottles (Langdon SP et al 2004) this is achieved by using all the internal surface for cell growth, rather than just the bottom of a bottle. The added advantages are that: a smaller volume of medium and thus a higher product titre can be achieved; the cells are more efficiently oxygenated due to alternative exposure to medium and the gas phase; and dynamic systems usually generate higher unit cell densities than stationary systems.