Complete Genome Sequence of Multi-Drug Resistant Klebsiella Quasipneumoniae Isolated for The First Time from A Wastewater Treatment Plant in Slovenia

Abstract

Jose Goncalves, Tom Koritnik. Martin Bosilj, Verica Mioc, Marija Trkov, and Metka Paragi

Objectives: This study reports the isolation and genomic characterization of two novel multidrug-resistant isolates of Klebsiella quasipneumoniae, isolated from a municipal wastewater treatment plant in Slovenia. Methods: Both genomes were sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using Velvet 1.2.10. The genomes were annotated using RAST tool kit (RASTtk). The resistome was identified using tools available from the Center for Genomic Epidemiology. Results: High-quality contigs were obtained using and both belong to the MSLT ST 138. KB-61219-C generated 196 contigs and an estimated genome length of 5.958.270 bp. KB-161219-D generated 197 contigs and an estimated genome length of 5.955.847 bp. Conclusion: This study reports the isolation and genomic characterization of two novel multidrug-resistant isolates of Klebsiella quasipneumoniae, isolated from a municipal wastewater treatment plant in Slovenia.

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