Biochemistry Characterization of Type 2 Diabetes in Tunisia and their Relationship with 4545G/C Polymorphism Located in Adiponectin Gene

Abstract

Hajer Kilani, Ameni Kallel and Riadh Jemaa

Diabetes incidence is increasing worldwide. Many studies demonstrated that polymorphisms within the adiponectin gene could be associated with type 2 diabetes mellitus (T2DM). A case–control study was conducted to find the association between SNP 4545G/C and T2DM in Tunisian population. The study included 561 patients referring to diabetic and 437 healthy con- trols. DNA was extracted from blood and genotyped by PCR–RFLP by using (HinfI) enzyme. Prevalence of obesity (p <0.001), dyslipidemia (p <0.001), hypertension (p <0.001) were significantly increased in cases with report to witnesses. Also for the lipid parameters Urea, CRP and HDL-C were significantly (p <0.001) and HDL / LDL (p = 0.983) are decreased in diabetics compared to controls two types of alleles G (Absence of restriction site) and C (presence of the restriction site) respectively of frequencies (0.76 and 0.23) in the controls and (0.73 and 0.26) in the diabetics, as well as three genotypes GG, GC and CC In comparison with the GG genotype the relative risk (OR [95% CI]) for diabetes was 1.06 [0.86-1.31]; p = 0.275 for the genotypes (GC + CC) . Our results show significant variations for the anthropometric and lipid parameters in the type 2 diabetes mellitus (T2DM) but were not associated with the polymorphisms at site of 4545.

PDF