Assessments of Antibacterial Photo-Dynamic Inhibition Technique (Apdt) In Combating Multiple Drug Resistant Clinical Isolates (MDRCI)

Abstract

Oludare Temitope Osuntokun, Thonda Oluwakemi Abike and Ajana KamaldeenOlamilekan

The purpose of this research is to assess the effect of Antibacterial photo-dynamic inhibition technique(APDT) in combating multiple resistance clinical isolates (MDRCI). Theera of antibiotic resistance opened new doors for scientists in the discovery of new antibiotics and new more chemotherapeutic agents, these discoveries leads to easy treatment of variety of bacterial infection. For this study, multidrug resistance isolates of Vibrio cholera and Staphylococcus aureus and susceptible strain of Staphylococcus epidermidiswere used for this research work. All isolates were from clinical sources. Riboflavin (vitamin B2 ) was used as photo-sensitizer at different logarithmic concentrations ranging from 100 to 104 , while the light source was from a UV foto-dyne documentation effecting photons at 300nm. There were controls for all the pathogens used and grouped as blank, light and riboflavin exposed organism were the main focus for this study. It was observed that those treated with UV light and riboflavin at various concentrations below 100 showed successful inhibition effects on all the clinical pathogenic isolates assayed at various light exposure. It was also observed that the control group showed different low responses compared with those treated with UV light, the clinical isolates treated with UV light and riboflavin has high response and considered to be relatively reactive asphoto-dynamic inhibitors. In conclusion, antimicrobial photo-dynamic inhibition therapy may be a useful tools in the inhibition of clinical isolates as modern tools for the eradication of resistance microorganism.

PDF