Anti-Spike Antibody Responses to Covid-19 Vaccine 3 Doses in Health Care Workers Working in Acute Care Hospital in Myanmar

Abstract

Khin Phyu Pyar, Zar Ni Htet Aung, Sai Aik Hla, Soe Win Hlaing, Soe Min Aung, Nyan Lin Maung, Thein Tun My-int, Kyaw Thet Maung, Aung Thu, Han Lin Aung, Thurein Wynn, Aung Phyo Kyaw, Saw Thar Wah, Myo Thant Kyaw, Zay Phyo Aung, Sit Min3, Myat Ko Ko, Kaung Myat Oo, Htet Aung4, Thi Han Tun

Background: The health care workers are prone to COVID-19 infection as they are working in front line; thus, they are in priority groups for vaccination. This study aimed to assess the level of anti-Spike antibody 2 weeks after 3 doses of COVID-19 vaccine among health care workers (HCW).

Methods: A cross-sectional descriptive study was conducted in July 2022 among health care workers (HCW) who received COVID-19 vaccine 3 doses. Data were collected by using standardized forms and analysis was done.

Results: A total of 42 HCW were included; the mean anti-Spike antibody level was 3734.19 U/mL. Female had higher anti-Spike antibody level than male, 4857.67 U/mL and 3427.78 U/mL respectively. HCW with diabetes mellitus had significantly higher anti-Spike antibody level 6740.00 U/mL than those without diabetes mellitus 2884.00 U/mL. Anti-Spike antibody level in smokers (3376.42 U/mL) was lower than that of non-smoker (3845.99 U/mL). HCW with history of COVID-19 infection had higher anti-Spike antibody level (4013.79 U/mL) than that of those without infection (3524.48 U/mL); those with history of COVID-19 infection in fourth wave (The Omicron outbreak; 6 months ago) had higher antibody level (4013.79 U/mL) than that of those with history of infection in third wave only (The Delta outbreak; one year ago) (3524.48 U/mL). HCW who got vaccinated in the afternoon had higher antibody level (4350.77 U/mL) than who got in the morning (2912.07 U/mL). Negative relation was detected between time from last vaccination to anti-Spike protein antibody level though it was not statistically significant. Significant predictors for anti-Spike antibody level on univariable analysis were BMI and presence of diabetes.

Conclusions: Anti-Spike antibody level was significantly related with BMI and diabetes mellitus; those with high BMI and diabetes mellitus had higher level of antibody. Anti-Spike antibody level was relatively higher in female; non-smokers; those with COVID-19 infection particularly in fourth wave (The Omicron infection); those with shorter duration from last vaccination; and those who got vaccination in the afternoon although it was not significant statistically.

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