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Journal of Oral & Dental Health(JODH)

ISSN: 2573-8224 | DOI: 10.33140/JODH

Impact Factor: 1.5

Antimicrobial Activity of Angelica Dahurica Ethanol Extract Against Oral Bacteria

Abstract

Su-Mi Cha, Kyung-Yeol Lee, Sung-Mi Choi, Jeong-Hun Seo and Jeong-Dan Cha

Introduction: Angelica dahurica (A. dahurica) has the protective activity against dexamehasone-induced disorders, liver protective activity, antimutagenic activity, anti-inflammatory, anti-microbial, anti-oxidative, anti-asthmatic, and anti-cancer effects.

Aims of the Study: This study aimed to investigate the synergistic antibacterial activity with existing antimicrobial agents against oral pathogen.

Materials and Methods: The synergistic effects of 50% ethanol extract of A. dahurica (ADEE) were evaluated against oral bacteria, either alone or with antibiotics, via broth microdilution and time-kill method.

Results: The minimal inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) values for ADEE, ampicillin and gentamicin against all the tested bacteria ranged between 62.5-1000/250-2000 μg/mL, 0.0625-16/0.25-32 μg/mL, and 4-128/16-256 μg/mL, respectively. The ADEE displayed synergism with ampicillin and gentamicin, with 8-fold reductions in the MIC/MBC. Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after treatment with 1/2 MIC of ADEE with 1/2 MIC of antibiotics resulted from an increase of the rate of killing in units of CFU/mL to a greater degree than was observed with alone.

Discussion and Conclusions: The results of this study demonstrate the antimicrobial and synergistic activity of ADEE and antibiotics against oral pathogens.

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