Aloe vera gel conjugated cuttlefish bone graft material treated with three types of hydroxyapatite for clinical trial

Abstract

Dong-Myong Kim, Hyung-Kon Lee, Yong-Seong Kwon, Yeon-Mea Choi

We have developed three types of hydroxyapatite particle (HP) functionalized Aloe vera gel W (AGW®) conjugated cuttlefish bone graft materials (Aloe-CGMs) for clinical applications. These Aloe-CGMs were prepared by attaching platelet, needle, and sphere shaped HPs to the surface of cuttlefish bone graft materials through chemically AGW® conjugation. Although all three HPs contributed to increase the surface area of graft material (GM), the shape of the HPs was a determining factor. Platelet HPs were most effective, because they caused a 62.3% increase in GM surface area whereas the influence of the spherical particle was only a 5.2% increase. This suggests that geometric factors regarding both the attached HPs and graft material surface are essential in controlling the surface roughness of graft materials. Among the three HPs, it was the platelet HPs that helped to increase the efficacy of the GM most significantly. Compared with GM with no HP attachment, Aloe-CGMs with platelet shaped HP (platelet Aloe-CGMs) treatment had ~58.9% higher cell attachment and proliferation rate. The MTT assay also showed that the HP treated cuttlefish bone graft materials had negligible cytotoxicity

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