A Review on Diagnostic Potentials of Undecalcified Tissue Sections in Histopathology

Abstract

Favour Chinwendu Ohiri, Yibala Ibor Oboma, Bassey Okon Ekpenyong, Philip Terkimbi Agbe, Owajioniro Godstime Mordecai and Esther Bereni Ene

The preparation of undecalcified tissue sections is essential for accurate histopathology evaluation of mineralized tissues. The preparation involves fixation, embedding, sectioning processes, and special staining techniques, their principles tailored for hard tissues such as Von Kossa method, Alizarin Red S method, alongside a comprehensive overview of the materials and equipment required. Frequently requested tissue types include; bone biopsies, dental samples and mineralized cartilage, often analyzed in the context of condition such as osteoporosis, dental caries, and Osteosarcoma. Despite the advantages, the technique has limitations, including the need for specialized training requirement, specialized equipment and expertise, as well as potential challenges in achieving uniform sectioning. Overall, this paper explores the importance of undecalcified section preparation in advancing histopathology research and clinical diagnosis, paving the way for enhanced understanding of bone-related conditions.

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