In vitro metabolic stability of nine fragrance chemicals was evaluated in trout and human hepatocytes. Compound disappearance with time was observed using gas chromatography/mass spectrometry. in vivo hepatic intrinsic clearances were calculated from the in vitro data. Significant metabolism was observed with trout hepatocytes for five of the nine chemicals, while all nine were metabolized significantly with human hepatocytes. Calculated half‐lives are shorter for humans than trout. For all chemicals with demonstrated hepatic metabolism, the models indicate an absence of accumulation. this study explores the detoxification effect of Retro‐2 on albumin (RT) cytotoxicity, moreover because the mechanisms underlying such effects. The RAW264.7 cells was wont to evaluate the detoxification effect of Retro‐2 on RT. The results indicated that cell viability, capacity for protein synthesis and release levels of the many cytokines were higher, while the expression levels of cytokine, or endoplasmic reticulum stress (ERS)‐related mRNA, were lower in cells pretreated with 20μm Retro‐2 and challenged with RT, compared with those who had not been pretreated with Retro‐2. finally, Retro‐2 retained the capacity for protein synthesis inhibited by RT, alleviated ERS induced by RT and increased the viability of cells challenged with RT. Retro‐2 shows the potential for clinical applications. Metabolomics combined with conventional methods were wont to explore cantharidin (CTD)‐induced hepatotoxicity in rat. Using serum and liver metabolomics, 4 biomarkers within the serum and 15 within the liver were found to be related to CTD hepatotoxicity. The hepatotoxicity of CTD appears to involve three metabolic pathways: glycerophospholipid metabolism, sphingolipid metabolism, and steroid biosynthesis.