In scientific research, tissue culture refers to a way during which fragments of a tissue are introduced into a replacement, artificial environment, where they still function or grow. While fragments of a tissue are often used, it's important to notice that entire organs also are used for tissue culture purposes. Here, such growth media as broth and agar are wont to facilitate the method. While the term tissue culture could also be used for both plant and animal tissues, plant part culture is that the more specific term used for the culture of plant tissues in tissue culture. Embryo culture is that the sort of tissue culture that involves the isolation of an embryo from a given organism for in nitro growth. Embryo culture may involve the use of a mature of immature embryo. Whereas mature embryos for culture are essentially obtained from ripe seeds, immature embryo involves the use of immature embryos from unripe/hybrid seeds that failed to germinate. In doing so, the embryo is ultimately able to produce a viable plant. For embryo culture, the ovule, seed or fruit from which the embryo is to be obtained is sterilized, and therefore the embryo does not have to be sterilized again. Salt sucrose may be used to provide the embryo with nutrients. The culture is enriched with organic or inorganic compounds, inorganic salts as well as growth regulators. Callus - This is the term used to refer to specialized, unorganized and a dividing mass of cells. A callus is produced when ex plants (cells) are cultured in an appropriate medium - an honest example of this is often the tumor tissue that grows out of the injuries of differentiated tissues/organs. In practice, callus culture involves the growth of a callus, which is the followed by a procedure that induces organ differentiation. For this sort of tissue culture, the culture is usually sustained on a gel medium, which consists of agar and a mix of given macro and micro nutrients counting on the type of cells. Different types of basal salt mixtures like Murasaki and scowl medium also are utilized in addition to vitamins to reinforce growth