Synthetic biology is currently the most commonly accepted definition, and is used in various ways depending on the discipline or specific use. Because it is an emerging area of ​​research, several fields of study are used and many definitions can be found in the literature. However, all the definitions touch on a common concept: the creation of new biological systems via synthesis or assembly of artificial or natural components. Biological systems are thus assembled by module. Cell-free protein expression systems are often used as membrane-based molecular machines.  More and more efforts are being made to bridge the gap between these two forming hybrid living / synthetic cells, and the communication between populations of living and synthetic cells. Several new enabling technologies are essential to the success of synthetic biology. Concepts include biological parts of standardization and the use of hierarchical abstraction in these parts of synthetic systems. Basic technologies include reading and writing DNA (sequencing and manufacturing). Multiple conditions under measurements are required for precise modeling and computer-aided design