The SCDt is based on the rule that sperm with divided DNA falls flat to deliver the characteristic radiance of scattered DNA circles that are watched in sperm with nonfragmented DNA, taking after corrosive denaturation and expulsion of atomic proteins. The SCDt recognizes cells with intaglio DNA (expansive radiance) from sperm cells with harmed DNA (little or missing radiance). Among the DNA astuteness tests specified over, the TUNEL and SCSA are the foremost commonly utilized so distant; be that as it may, the SCDt was presented as a straightforward, quick, precise, and exceedingly reproducible strategy for the investigation of sperm DNA fracture in semen and prepared sperm. In expansion, the SCDt does not require the utilize of complex instrumented; it can be carried out with gear regularly accessible in andrology research facilities (i.e., light magnifying instruments), and the test endpoints (nondispersed and scattered cores) can be effectively surveyed by research facility professionals