Cutting edge sequencing (NGS) is otherwise called high-throughput sequencing it is term used to depict various diverse current sequencing advancements including Illumina (Solexa) sequencing, Whole genome sequencing, Targeted sequencing, Amplicon sequencing, exome sequencing, De novo sequencing , Transcriptomics and so forth. These ongoing innovations permit us to grouping DNA and RNA substantially more rapidly and inexpensively than the recently utilized Sanger sequencing, and as such have changed the investigation of genomics and sub-atomic science.