The developing utilization of counter acting agent based partition techniques has resembled the extension of immunochemical identification strategies in moving past the clinical indicative field to applications in ecological checking. As of late elite immunoaffinity chromatography, which started as a partition procedure in biochemical and clinical research, has been adjusted for isolating and measuring natural poisons. Bio affinity offers a particular natural reason for partition that can be fused into a measured expository procedure for increasingly proficient ecological investigation. The utilization of immunoaffinity chromatography for partition supplements the utilization of immunoassay for identification. A generally utilized immunochemical identification strategy for ecological examinations is compound immunoassay. The goal of this paper is to audit the status of bio affinity-based explanatory methods for ecological applications and human introduction evaluation considers. Natural strategies dependent on bio affinity go from develop immunoassays to rising methods, for example, immunosensors and immunoaffinity chromatography techniques for little atoms. The interest for robotization of fluid extraction (LLE) in sedate examination joined with the interest for decreased example planning time has prompted the on-going advancement of fluid stage micro extraction (LPME) in view of expendable empty strands. In LPME, target drugs are extricated from fluid natural examples, through a slim layer of natural dissolvable immobilized inside the pores of the mass of a permeable empty fibre, and into a μl volume of acceptor arrangement inside the lumen of the empty fibre. After extraction, the acceptor arrangement is oppressed legitimately to a last investigation either by superior fluid chromatography (HPLC), slim electrophoresis (CE), mass spectrometry (MS), or hair like gas chromatography (GC) with no further medicines. Empty fibre-based LPME may give high advancement of medications and great example tidy up, and most likely has a wide application potential inside the zone of medication investigation. This audit centres on the rule of LPME, and late utilizations of three-stage, two-stage, and transporter interceded LPME of medications from plasma, entire blood, pee, and bosom milk.