Peptidomics is the study of soluble native peptides and little proteins found in body fluids, primarily plasm and CSF. Invaluable data are often gleaned from activity of those varied soluble polypeptides, several of those square measure communication molecules like cytokines, growth factors and amide hormones. There are multitude of alternative tiny macromolecule and amide fragments, several with undetermined operate and plenty of ensuing from disease-specific chemical action cleavage of alternative current proteins or cell surface associated proteins. These soluble fragments will give a wealth of knowledge concerning illness state, drug efficaciousness or toxicity and since the peptidomics methodology removes 99% of hyper-abundant proteins before analysis its matchless sensitivity in biomarker discovery.