Next generation sequencing alludes to procedures in which a large number of nucleotide successions are deciphered all the while (explored by Goodwin and colleagues210) and is frequently called greatly equal sequencing. The total sequencing of the human genome during the mid 2000s192–194 incited a requirement for generally modest, high-throughput sequencing strategies, a need that was met by a few NGS NGS stages have demonstrated to be exceptionally quantitativ and versatile for multiplex marker testing to meet explicit clinical needs. These stages require a stepwise way to deal with tissue handling, nucleotide extraction, and library groundwork for effective biomarker testing.