Next generation sequencing (NGS), hugely equal or profound sequencing are connected terms that portray a DNA sequencing innovation which has altered genomic research. Utilizing NGS a whole human genome can be sequenced inside a solitary day. Conversely, the past Sanger sequencing innovation, used to unravel the human genome, required longer than 10 years to convey the last draft. Despite the fact that in genome research NGS has generally supplanted ordinary Sanger sequencing, it has not yet converted into routine clinical practice. The point of this article is to survey the likely uses of NGS in pediatrics. Technology, Basic Science.There are various NGS stages utilizing diverse sequencing advancements, an itemized conversation of which is past the extent of this article. Be that as it may, all NGS stages perform sequencing of a large number of little pieces of DNA in equal. Bioinformatics examinations are utilized to bits together these sections by mapping the individual peruses to the human reference genome. Every one of the three billion bases in the human genome is sequenced on various occasions, giving high profundity to convey exact information and a knowledge into sudden DNA variety . NGS can be utilized to arrangement whole genomes or obliged to explicit zones of enthusiasm, including all 22 000 coding qualities (an entire exome) or little quantities of individual qualities.