Next-generation sequencing is defined as a process where DNA strands are sequenced parallel and substantially minimize need for the fragment cloning which are used in sanger sequencing of genome. There are different technologies used in next-generation sequencing. The high demand for low-cost sequencing has driven the development of high-throughput sequencing, which is also termed as Next-generation sequencing (NGS). Thousands or millions of sequences concurrently produced in next-generation sequencing process. Next-generation sequencing has become a commodity. Sometimes, an article describes a new tool or method. Because articles in scientific journals are specific, meticulously cited and peer-reviewed, journal databases are the best place to look for information on previous research on your species. Without a background in the field, journal articles may be hard to understand - however, you do not need to understand an entire article to be able to get valuable information from it. Existing sequencing technologies are more mature and accessible than the methodologies that are available for individual researchers to move, store, analyse and present data in a fashion that is transparent and reproducible.