The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Journal of Next Generation Sequencing & Applications is a broad-based journal found on two key tenets: To publish the most exciting Reviews on Next generation Sequencing & Applications: Second to provide a rapid turn-around time possible for reviewing and publishing of articles freely for research, teaching and reference purposes.It is basically aimed at the Clinical Practitioners, medical/ health practitioners, students, professionals and researchers and professional bodies and institutions. Given that sequencing-based assays may increasingly supersede microarray-based assays, we also compare and contrast data obtained from these distinct approaches. additional tools to aid QC (sequence format converter and trimming tools) and analysis (statistics tools). A variety of options have been provided to facilitate the QC at user-defined parameters. The toolkit is expected to be very useful for the QC of NGS data to facilitate better downstream analysis.