This procedure is fundamentally used to decide the transformations in any creatures, assuming any. The essential methodology is that the preliminaries that are immobilized arrive at the format and strengthen to them by the utilization of dideoxynucleoside. They are mostly present at a place that is neighboring the freak nucleotide. The open access diaries are peer audited academic diaries of Next Generation: Sequencing and Application. The top open access diaries are unreservedly accessible on the open web area, permitting any end clients to peruse, download, duplicate, disseminate, prink, search or connection to the full messages of the articles. These give high caliber, fastidiously audited and quick distribution, to provide food the obstinate need of academic network. These diaries are filed with every one of their references noted. The top open access diaries are filed in SCOPUS, COPERNICUS, CAS, EBSCO and ISI.