Specimens square measure collected with a blade or by mopping the concerned areas of the skin. once pustules or vesicles square measure gift, the roof or crust is removed with a sterile surgical blade. The pus or exudate is unfold as thinly as attainable on a transparent glass slide for Gram staining. microorganism infection is that the combinatorial method of invasion of infectious agents into the organism, their multiplication and therefore the reaction of host tissue against these agents. Infectious agents chiefly embrace bacterium, virus, parasite, fungi . Primary Infections: Primary skin infections have a characteristic clinical image and illness course, square measure caused by one infectious agent, and frequently have an effect on traditional skin. Impetigo, rubor, and boils square measure common varieties. the foremost common primary skin pathogens square measure S aureus, β-hemolytic streptococci, and coryneform bacterium. These organisms sometimes enter through a possibility within the skin like associate degree injury. several general infections involve skin symptoms caused either by the infectious agent or by toxins; examples square measure morbilli, varicella, gonococcemia, and cocci scalded skin syndrome. Dermatophytic fungi have a robust affinity for ceratin and thus invade keratinized tissue of the nails, hair, and skin. Microbial infection journals aims to publish innovative scientific content such as research papers, case reports, review articles, short communications, commentaries to name a few. It is the basic research article, applied research article, or translational researches conducted to aid and support the evolution of knowledge in the field of Healthcare.