A story, explicit and delicate single-atom checking (SIM) gas chromatography-mass spectrometry (GCMS) methodology was made and embraced for the attestation of centrality metabolites identified with glycolysis, the tricarboxylic ruinous (TCA) cycle, glutaminolysis, and unsaturated fat β-oxidation. The appraisal was utilized to eviscerate liver tissue and serum from C57BL/6N solid mice managed a high-fat eating routine (HFD) and C57BL/6N mice managed ordinary chow for around two months. HFD-managed mice serum demonstrated really completely lessened groupings of pyruvate, citrate, succinate, fumarate, and 2-oxoglutarate, with a raised blending of pantothenic dangerous. In liver tissue, HFD-managed mice showed incapacitated degrees of glycolysis indisputable results pyruvate and lactate, glutamate, and the TCA cycle intermediates citrate, succinate, fumarate, malate, and oxaloacetate. Pantothenate levels were 3-overlay raised joined by an unassuming expanded quality articulation of Scl5a6 that encodes the pantothenate transporter SLC5A6. Since both glucose and unsaturated fats limit coenzyme A relationship from pantothenate, it was accepted that these information were steady with downregulated unsaturated fat β-oxidation, glutaminolysis, glycolysis, and TCA cycle improvement, because of baffled anaplerosis. The epic SIM GCMS take a gander at gave new bits of data into metabolic impacts of HFD in mice.