The lymphatic and blood systems are interconnected—extracellular fluid flows from vascular capillaries into lymphatic microvessels and is returned to the vascular system via the lymphatic vessel. However, while much is understood about the biology of the blood system, the biology behind the event and regulation of the systema lymphaticum remains comparatively poorly understood. Until recently, the amount of molecular tools that allowed us to differentiate between blood and lymphatic vessels and cells within tissues was limited. However, in recent years researchers are ready to distinguish quite definitively between the tissues of the 2 systems at the molecular level. This ability to discriminate between cell types allows for the simple isolation of pure lymphatic cell cultures, and successively the flexibility to check lymphatic biology in greater detail. A macroscopic observe the vessels of the cardiovascular and lymphatic systems makes it obvious that there are structural and organizational similarities. this is often partly because of the connection between the function of the 2 systems. Both systems contain large networks of vessels, the vessels in both systems are composed of smooth it obvious that there are structural and organizational similarities. this is often partly because of the connection between the function of the 2 systems. Both systems contain large networks of vessels, the vessels in both systems are composed of smooth muscle walls lined with a monolayer of endothelium, and that they both target complexity from larger multi-layered vessels to capillaries composed mostly of endothelium. However, the differences between the 2 also correlate with their function and are evident at the structural, cellular, and molecular levels. Such similarities and differences make an effect on how closely the tissue engineering strategies for every system should correlate.