Loop-mediated equal amplification (LAMP) may be a single-tube technique for the amplification of DNA[1][2] and a low-priced different to notice bound diseases. Reverse Transcription Loop-mediated equal Amplification (RT-LAMP) combines LAMP with a reverse transcription step to permit the detection of RNA.
LAMP is associate degree equal supermolecule amplification technique. In distinction to the polymerase chain reaction (PCR) technology, during which the reaction is distributed with a series of alternating temperature steps or cycles, equal amplification is distributed at a continuing temperature, and doesn't need a thermal cycler.
"LAMP" stands for Loop-mediated equal Amplification. This technology was developed by Notomi et al. it's a really sensitive, simple and time economical technique. The LAMP reaction takings at a continuing temperature employing a strand reaction.
We have developed a unique technique, termed loop-mediated equal amplification (LAMP), that amplifies DNA with high specificity, potency and rate below equal conditions. This technique employs a DNA enzyme and a collection of 4 specially designed primers that acknowledge a complete of six distinct sequences on the target DNA. associate degree inner primer containing sequences of the sense and antisense strands of the target DNA initiates LAMP. the subsequent strand displacement DNA synthesis ready by associate degree outer primer releases a fiber DNA. This is model for DNA synthesis ready by the second inner and outer primers that hybridise to the opposite finish of the target, that produces a stem–loop DNA structure. In ensuant LAMP athletics one inner primer hybridizes to the loop on the merchandise and initiates displacement DNA synthesis, yielding the first stem–loop DNA and a brand new stem–loop DNA with a stem double as long. The athletics reaction continues with accumulation of 109 copies of target in but associate degree hour. the ultimate product square measure stem–loop DNAs with many inverted repeats of the target and cauliflower-like structures with multiple loops shaped by hardening between alternately inverted repeats of the target within the same strand. as a result of LAMP acknowledges the target by six distinct sequences ab initio and by four distinct sequences after, it's expected to amplify the target sequence with high property.