Immobilization of the whole cell offers high metabolic activity and Productivity. It is one of the key methods for the improvement of microbial strains producing bioactive compounds. In the present study, 18 actinomycete isolates were isolated from garden soil and screened regard with their potential against both gram-positive and negative bacteria including MRSA. Among the 18 strains, KUA06 showed good activity and they were characterized by macroscopically and microscopically. The promising isolate KUA06 were cultivated in liquid culture medium and immobilized with sodium alginate. The antibacterial compound was extracted by solvent extraction technique. Comparative studies on the total antibiotic sensitivity of the free cells and immobilized cells showed that the immobilized cells were effective against test pathogens; 25 mm against medically important organisms. Further, we concluded that the isolate immobilized KUA06 are more efficient for antibiotic synthesis against multidrug pathogens. Enzyme immobilization is a technique specifically designed to restrict the freedom of movement of an enzyme. Immobilization of enzymes is a common practice, mainly in order to minimize enzyme costs on the process economics by making it possible to reuse the enzyme many times and also minimize the operation cost as the immobilization technique may be modify the enzyme behaviour, thus reducing the enzyme and product costs significantly. Many techniques have been used previously for enzyme immobilization, as entrapment, adsorption, covalent binding, encapsulation, and cross linking. Here we compare and contrast the basic properties of all immobilization methods. Traditionally, four methods are used for enzyme immobilization, namely (1) non-covalent adsorption and deposition, (2) physical entrapment, (3) covalent attachment, and (4) bio-conjugation.