Bioinformatics encompasses the development and application of software tools to assist the understanding of biological functions and data, while systems biology involves mathematical and computational modelling of biological systems and functions for simplified representation, understanding and documentation. Bioinformatics incorporates and applies the theoretical and practical knowledge of statistics, mathematics, computer science, engineering and biology and allows in silico analysis of biological data and computerised interpretation of that data for future applications. On the other hand systems biology utilises the persisting knowledge on functioning of signalling pathways, metabolic networks and genetic sequences for the furtherance of scientific research and application. The Human Genome Project is the most important contribution of systems biology in the last hundred years