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Enzyme Activity Best Online Journals

The specific activity of associate degree accelerator is another common unit. this will be the activity of associate degree accelerator per weight unit of total molecule (expressed in μmol min−1 mg−1). Specific activity provides a activity of accelerator purity at intervals the mixture. it is the tiny moles of product intentional by associate degree accelerator throughout a given amount of it slow (minutes) below given conditions per weight unit of total proteins. Specific activity is adequate the speed of reaction hyperbolic by the amount of reaction divided by the mass of total molecule. The SI unit is katal/kg, but a extra wise unit is μmol/mgmin.
Specific activity can be a live of accelerator processivity (the capability of accelerator to be processed), at a specific (usually saturating) substrate concentration, and is usually constant for a pure accelerator.
An active computer meter analysis methodology area unit typically in quandary the elimination of errors arising from variations in cultivation batches and/or misfolded accelerator and similar issues. this will be a live of the amount of active accelerator, calculated by e.g. titrating the amount of active sites gift by mistreatment associate degree irreversible matter. the actual activity need to then be expressed as μmol min−1 mg−1 active accelerator. If the relative molecular mass of the accelerator is believed, the turnover selection, or μmol product per second per μmol of active accelerator, area unit typically calculated from the actual activity. The turnover selection area unit typically pictured as a result of the range of times each accelerator molecule carries out its activity cycle per second.

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Last Updated on: Jul 04, 2024

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