Two anthraquinonic colors, C.I. Corrosive Blue 225 and C.I. Corrosive Violet 109, were utilized as models to investigate the possibility of utilizing the horseradish peroxidase catalyst (HRP) in the down to earth decolorization of anthraquinonic colors in wastewater. The impact of procedure boundaries, for example, chemical fixation, hydrogen peroxide focus, temperature, color focus, and pH was inspected. The pH and temperature movement profiles were comparative for decolorization of the two colors. Under the ideal conditions, 94.7% of C.I. Corrosive Violet 109 from fluid arrangement was decolorized (treatment time 15 min, chemical focus 0.15 IU/mL, hydrogen peroxide fixation 0.4 mM, color fixation 30 mg/L, pH 4, and temperature 24°C) and 89.36% of C.I. Corrosive Blue 225 (32 min, catalyst fixation 0.15 IU/mL, hydrogen peroxide focus 0.04 mM, color fixation 30 mg/L, pH 5, and temperature 24°C). The component of the two responses has been demonstrated to follow the two substrate ping-pong system with substrate restraint, uncovering the development of an inefficient or impasse complex among color and HRP or among H2O2 and the oxidized type of the chemical. Both compound oxygen request and absolute natural carbon esteems demonstrated that there was a decrease in harmfulness after the enzymatic treatment. This examination confirms the suitability of utilization of horseradish peroxidase for the wastewaters treatment of comparable anthraquinonic colors.