Both in civil and criminal cases DNA analysis has become as an important tool to solve cases. In crime scene, evidences like hair, skin, body remains in burnt cases, semen in rape cases etc. will be analysed for DNA matches. In cases such as paternity confirmation also, DNA analysis is being used. This filed of science is Forensic DNA analysis.Discrimination of one being to another with the help of hypervariable minisatellite deoxyribonucleic acid (DNA) is forensic DNA analysis. Using PCR, several DNA profiles have been created and stored in developed countries for further forensic DNA analysis. These forensic DNA analysis will be further used to compare evidences and prove crimes. Genetic engineering has a very good role in forensic DNA analysis.