Hepatitis B infection (HBV) contamination is a significant worldwide medical issues prompting serious liver malady, for example, cirrhosis and hepatocellular carcinoma (HCC). HBV is a roundabout, somewhat twofold abandoned DNA infection with different serological markers: hepatitis B surface antigen (HBsAg) and enemies of HBs, hostile to HBc IgM and IgG, and It is transmitted by sexual, parenteral and vertical course. One huge strategy to decrease the weight of this illness is opportune conclusion of intense, constant and mysterious instances of HBV. Initial step of HBV finding is accomplished by utilizing serological markers for identifying antigens and antibodies. So as to confirm initial step of conclusion, to evaluate viral burden and to distinguish genotypes, quantitative or subjective sub-atomic tests are utilized.In this article, the serological and sub-atomic tests for determination of HBV contamination will be assessed.