Cryo-preservation or cryo-conservation may be a method wherever organelles, cells, tissues, living thing matrix, organs, or the other biological constructs prone to injury caused by unregulated chemical Biological and chemical reactions in living cells area unit dramatically reduced at temperature, a development that may cause the potential long preservation of cells and tissues. However, state change is fatal to most living organisms, since each intra- and living thing ice crystals area unit fashioned and leads to changes to the chemical setting of cells that cause cellular mechanical constraints and injury. The CPA, that is sometimes a fluid, reduces the state change injury from the cryopreservation method . CPAs ought to be biologically acceptable, be ready to penetrate the cells, and have low toxicity.2 numerous CPAs are developed and area unit wont to cut back the quantity of ice fashioned at any given temperature, betting on the cell sort, cooling rate, and warming rate.