The incremented sensitivity and availability of NAATs for detection of virtually any clinically germane virus have revolutionized testing in the clinical virology laboratory. In many laboratories, NAATs have supplanted virus culture, antigen detection methods, and direct molecular hybridization techniques. Three approaches have been taken target amplification such as PCR, strand displacement amplification, nucleic acid sequence based amplification, and transcription-mediated amplification systems; probe amplification, including; replicase and ligase chain reaction; and signal amplification, such as branched-chain DNA (bDNA) assay and hybrid capture assay. Several commercial and in-house assays have been developed. Quantification of viral genome in plasma or serum can be acclimated to determine prognosis, cull patients for antiviral therapy, and monitor replication to treatment in a variety of patient populations. Multiplex assays capable of detecting several viruses in a single amplification reaction have been developed and studies suggest that these assays are cost-efficacious. The development of automated genuine-time PCR utilizing fluorescence techniques and perpetual detection of amplified product has abbreviated detection times substantially relative to conventional PCR assays. Because these assays utilize a closed system they withal are less prone to contamination than conventional PCR. NAAT has been applied to genotyping of viruses, as well as for the detection of mutations that confer resistance to antiviral agents. More incipient molecular techniques such as pyrosequencing and whole-genome sequencing are commencing to be applied in the clinical laboratory and are providing ameliorated genotyping of viruses, detection of virus quasispecies, and metagenomic analysis for detecting subsisting and incipient or novel viruses in clinical specimens.

To increase the visibility and ease of use of open access scientific and scholarly journals top online publishing journals are indexed in different indexing and archiving services. Indexing provides easy access of the article online. The top online publishing journals publish articles which are cited as references by many authors in their work. Citations are important for a journal to get impact factor. Impact factor is a measure reflecting the average number of citations to recent articles published in the journal. The impact of the journal is influenced by impact factor, the journals with high impact factor are considered more important than those with lower ones. Indexing provides easy access of the article online. The international journals are among the best open access journals in the world, set out to publish the most comprehensive, relevant and reliable information based on the current research and development on a variety of subjects. This information can be published in our peer reviewed journal with impact factors and are calculated using citations not only from research articles but also review articles editorials, letters, meeting abstracts, short communications, and case reports. The inclusion of these publications provides the opportunity for editors and publishers to manipulate the ratio used to calculate the impact factor and try to increase their number rapidly. Impact factor plays a major role for the particular journal.