A story, express and sensitive single-iota checking (SIM) gas chromatography-mass spectrometry (GCMS) strategy was made and grasped for the validation of centrality metabolites related to glycolysis, the tricarboxylic ruinous (TCA) cycle, glutaminolysis, and unsaturated fat β-oxidation. The examination was used to destroy liver tissue and serum from C57BL/6N strong mice dealt with a high-fat eating routine (HFD) and C57BL/6N mice oversaw common chow for around two months. HFD-oversaw mice serum showed actually totally reduced groupings of pyruvate, citrate, succinate, fumarate, and 2-oxoglutarate, with a raised mixing of pantothenic hazardous. In liver tissue, HFD-oversaw mice indicated debilitated degrees of glycolysis undeniable outcomes pyruvate and lactate, glutamate, and the TCA cycle intermediates citrate, succinate, fumarate, malate, and oxaloacetate. Pantothenate levels were 3-overlay raised joined by an unassuming extended quality enunciation of Scl5a6 that encodes the pantothenate transporter SLC5A6. Since both glucose and unsaturated fats limit coenzyme A relationship from pantothenate, it was acknowledged that these data were consistent with downregulated unsaturated fat β-oxidation, glutaminolysis, glycolysis, and TCA cycle improvement, in light of puzzled anaplerosis. The epic SIM GCMS look at gave new bits of information into metabolic effects of HFD in mice.